Journal:

Article Title: The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos 1 [w]

doi: 10.1104/pp.015214

Figure Lengend Snippet: Quality control of microarray experiments

Article Snippet: The purified DNA was denatured in 50% (w/v) dimethyl sulfoxide and spotted in four replicates onto Corning microarray technology-gamma amino propyl silane aminosilane-coated glass microscope slides (Corning, NY) by a 417 Arrayer (Affymetrix, Woburn, MA).

Techniques: Microarray

Journal: PLoS ONE

Article Title: Intra- and Inter-Individual Variance of Gene Expression in Clinical Studies

doi: 10.1371/journal.pone.0038650

Figure Lengend Snippet: Three kinds of samples were employed in this study. Individual variance was evaluated using the first sample group (G1), comprising Samples 1 to 9 of nine individuals. The second sample group (G2) was used to evaluate anatomic variance. It contained Samples 8–1, 8–2, and 8–3, taken from three different sections of placenta from the same individual. The third sample group (G3) consists of two technical replicates, Samples 8–3_1 and 8–3_2, using an identical RNA pool for microarray hybridization to evaluate technical variance. The expression of Sample 8–3 could be estimated by the mean expression of Samples 8–3_1 and 8–3_2. The mean expression of Samples 8–1, 8–2, and 8–3 represented the expression of Sample 8.

Article Snippet: A single microarray slide (CMT-GAPsII, Corning Inc., USA) contains 7334 human cDNA probes in quadruplicate, 10 spike-in genes (SpotReportTM-10 Array Validation System, Stratagene, USA), and one housekeeping gene, β-actin, in 96 replicates.

Techniques: Microarray, Hybridization, Expressing